rabbit polyclonal anti mmp3  (Proteintech)

Journal: Cell reports

Article Title: Breast cancer cell mesenchymal transition and metastasis directed by DAP5/eIF3d-mediated selective mRNA translation

doi: 10.1016/j.celrep.2023.112646

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-human/mouse MMP3 antibody , Abcam , Cat# ab52915/RRID:AB_881243.

Techniques: Recombinant, Staining, Protease Inhibitor, Virus, SYBR Green Assay, Bicinchoninic Acid Protein Assay, MTT Assay, Software, Imaging

Journal: Frontiers in Neuroscience

Article Title: Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for Glioma Migration and MMP3 Activation

doi: 10.3389/fnins.2019.00143

Figure Lengend Snippet: Secretome analysis identifies differentially expressed proteins. (A) Volcano plot of secretome proteins ( T -test P -value, and log 2 ratio) to identify differential expression. These proteins include reelin, MMP3 and osteopontin. Select MS/MS and MS spectra: (B) Reelin; (C) MMP3; and (D) Osteopontin showing identity and differential increases with C6/C6-13. (E) SDS-PAGE/Western blot of lysates and conditioned media (CM) supports increases in osteopontin in C6-13. (F) Table summarizing proteins found to be differentially expressed: fold-change ratio, P -value, anticipated cellular location, function/enzyme type and presence of a protein secretion signal.

Article Snippet: Membranes were incubated for 4 h with diluted rabbit polyclonal anti-MMP3 antibody (1:1000, 17873-1-AP, ProteinTech Group), or goat polyclonal anti-osteopontin (1:1000; Affinity BioReagents) antibodies in 5.0 mL of TBST containing 1% milk.

Techniques: Quantitative Proteomics, Tandem Mass Spectroscopy, SDS Page, Western Blot

Journal: Frontiers in Neuroscience

Article Title: Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for Glioma Migration and MMP3 Activation

doi: 10.3389/fnins.2019.00143

Figure Lengend Snippet: Binary increases of osteopontin and MMP3 in high motility C6-13. Targeted detection of (A) osteopontin/SPP1 and (D) MMP3 monitored by HR-MRM in biological triplicates of C6/C6-13 conditioned media. (B,E) Sequence, parent and fragment ions ( m/z ) used to monitor MMP3/osteopontin proteins. Significant increases of (C) osteopontin and (F) MMP3 are observed with C6-13 secretome ( ∗ p ≤ 0.05, ∗∗∗ p ≤ 0.001). (G,H) Fibronectin observed in C6/C6-13 was used to monitor sample load and signal normalization.

Article Snippet: Membranes were incubated for 4 h with diluted rabbit polyclonal anti-MMP3 antibody (1:1000, 17873-1-AP, ProteinTech Group), or goat polyclonal anti-osteopontin (1:1000; Affinity BioReagents) antibodies in 5.0 mL of TBST containing 1% milk.

Techniques: Sequencing

Journal: Frontiers in Neuroscience

Article Title: Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for Glioma Migration and MMP3 Activation

doi: 10.3389/fnins.2019.00143

Figure Lengend Snippet: MMP3 is active in the ECM of high motility glioma. (A) SDS-PAGE/Western blot demonstrates the binary increases of MMP3 in C6-13 vs. C6 glioma conditioned media (CM) in 3 biological replicates. (B) Increases in gelatinase activity associated with MMP3 is observed in the conditioned media of C6-13, but not C6 cells. (C) Cleavage of the MMP3-specific fluorgenic substrate (NFF-3) demonstrates increased proteolytic activity in C6-13 CM. Limited cleavage of NFF-3 is observed with C6. Fresh, unconditioned media served as a negative control for each time point. (D) Representative, quantitative values from the 2 h time point demonstrates robust increases in MMP3 activity with C6-13.

Article Snippet: Membranes were incubated for 4 h with diluted rabbit polyclonal anti-MMP3 antibody (1:1000, 17873-1-AP, ProteinTech Group), or goat polyclonal anti-osteopontin (1:1000; Affinity BioReagents) antibodies in 5.0 mL of TBST containing 1% milk.

Techniques: SDS Page, Western Blot, Activity Assay, Negative Control

Journal: Frontiers in Neuroscience

Article Title: Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for Glioma Migration and MMP3 Activation

doi: 10.3389/fnins.2019.00143

Figure Lengend Snippet: Pathways analysis of the 17 differentially regulated secretome proteins found in C6-13. Where possible, the activation Z -score functions of the ingenuity pathways tool was used to predict the biological impact of C6-13 expression.

Article Snippet: Membranes were incubated for 4 h with diluted rabbit polyclonal anti-MMP3 antibody (1:1000, 17873-1-AP, ProteinTech Group), or goat polyclonal anti-osteopontin (1:1000; Affinity BioReagents) antibodies in 5.0 mL of TBST containing 1% milk.

Techniques: Activation Assay, Expressing, Disruption, Migration, Binding Assay, Dispersion, Cell Function Assay, Clinical Proteomics, Membrane

Journal: Scientific Reports

Article Title: TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes

doi: 10.1038/srep42990

Figure Lengend Snippet: ( A ) Immunohistochemistry analysis of human cartilage tissues. Cartilage sections were obtained from normal and OA-affected cartilages. Sections were stained with MMP3 antibody. Left scale bars, 500 μm. Right scale bar, 200 μm. ( B ) MMP3 expression was detected by real-time qRT-PCR in human chondrocytes from 3 donors after Ad-GFP or Ad-TWIST1 infection at 72 hours. Values are the mean ± SEM of three independent experiments for each donor. ( C ) Relative MMP3 expression level in TC28 after Ad-GFP or Ad-TWIST1 infection. Values are the mean ± SEM. *P < 0.05, ***P < 0.001.

Article Snippet: Rabbit anti-human TWIST1 polyclonal antibody (Cell Signaling Technology), rabbit polyclonal anti-MMP3 antibody (Cell Signaling Technology), rabbit polyclonal anti-5hmC antibody (Active Motif) and mouse anti-TET1 antibody (GeneTex) were applied and incubated overnight at 4 °C.

Techniques: Immunohistochemistry, Staining, Expressing, Quantitative RT-PCR, Infection

Journal: Scientific Reports

Article Title: TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes

doi: 10.1038/srep42990

Figure Lengend Snippet: ( A ) Immunohistochemistry analysis of human cartilage tissues. Cartilage sections were obtained from normal and OA-affected cartilages. Sections were stained with 5hmC anti body. Left scale bars, 500 μm. Right scale bar, 200 μm. ( B ) Bisulfite genomic sequencing results of the MMP3 promoter regions in TC28 cells after Ad-GFP or Ad-TWIST1 infection at 72 hours. Black, methylated; white, unmethylated. ( C ) To detect locus-specific 5hmC and 5mC status at CCGG site in MMP3 promoter, restriction enzyme digestion and quantitative polymerase chain reaction were performed. *P < 0.05. ( D ) TET1, TET2, and TET3 expressions were detected by real-time qRT-PCR in human chondrocytes after Ad-GFP or Ad-TWIST1 infection at 72 hours. Values are the mean ± SEM of three independent experiments. *P < 0.05.

Article Snippet: Rabbit anti-human TWIST1 polyclonal antibody (Cell Signaling Technology), rabbit polyclonal anti-MMP3 antibody (Cell Signaling Technology), rabbit polyclonal anti-5hmC antibody (Active Motif) and mouse anti-TET1 antibody (GeneTex) were applied and incubated overnight at 4 °C.

Techniques: Immunohistochemistry, Staining, Genomic Sequencing, Infection, Methylation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes

doi: 10.1038/srep42990

Figure Lengend Snippet: ( A ) The siRNA for TET1 was transfected to human primary chondrocytes, and after 36 hours, cells were infected with Ad-GFP or Ad-TWIST1 and cultured for 72 hours. The MMP3 gene expression was determined by real-time qRT-PCR. ( B ) Fibroblasts were derived from Tet1, 2 and 3 triple knock out mice embryonic stem cells, and were transfected Ad-GFP or Ad-TWIST1 for 72 hours. Mmp3 gene expression was determined by quantitative PCR. *P < 0.05.

Article Snippet: Rabbit anti-human TWIST1 polyclonal antibody (Cell Signaling Technology), rabbit polyclonal anti-MMP3 antibody (Cell Signaling Technology), rabbit polyclonal anti-5hmC antibody (Active Motif) and mouse anti-TET1 antibody (GeneTex) were applied and incubated overnight at 4 °C.

Techniques: Transfection, Infection, Cell Culture, Gene Expression, Quantitative RT-PCR, Derivative Assay, Knock-Out, Real-time Polymerase Chain Reaction